DNA nanoball sequencing
DNA nanoball sequencing is a high throughput sequencing technology that is used to determine the entire genomic sequence of an organism. The method uses rolling circle replication to amplify small fragments of genomic DNA into DNA nanoballs. Fluorescent probes bind to complementary DNA and the probes are then ligated to anchor sequences bound to known sequences on the DNA template. The base order is determined via the fluorescence of the ligated and bound probes.
This DNA sequencing method allows large numbers of DNA nanoballs to be sequenced per run at lower reagent costs compared to other next generation sequencing platforms. However, a limitation of this method is that it generates only short sequences of DNA, which presents challenges to mapping its reads to a reference genome. The company Complete Genomics uses DNA nanoball sequencing to sequence samples submitted by researchers.[citation needed]

This is an excerpt from the article DNA nanoball sequencing from the Wikipedia free encyclopedia. A list of authors is available at Wikipedia.
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DNA nanoball sequencing - Wikipedia, the free encyclopedia
DNA nanoball sequencing is a high throughput sequencing technology that is used to determine the entire genomic sequence of an organism. The method uses ...
en.wikipedia.org/wiki/DNA_nanoball_sequencing
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Complete Genomics >> Technology
Our proprietary whole human genome sequencing technology consists of the ... two primary components of our sequencing technology: DNA nanoball arrays, ...
www.completegenomics.com/technology/
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Complete Genomics' Sequencing
Technology Overview—Complete Genomics' Sequencing | Complete Genomics, Inc. .... Additionally, because the DNA nano-ball (DNB™) production process.
core.iddrc.org/molecular-genetics/wp-content/complete_genomics_technology.pdf
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Next Generation Sequencing — Nanoball Sequencing | Wafergen
Oct 9, 2013 ... One of the newest ways to sequence in the realm of next generation sequencing is DNA nanoball sequencing. This new method guarantees ...
www.wafergen.com/next-generation-sequencing-nanoball-sequencing/
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BioTechniques - Sequencing's new race
These resulting DNA nanoballs (DNBs) consist of more than 200 copies of the head-to-tail concatemers in a ball-like configuration. The DNBs are then ...
www.biotechniques.com/BiotechniquesJournal/2010/February/Sequencings-new-race/biotechniques-187819.html?pageNum=4
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Watch DNA Nanoball Sequencing Video
DNA nanoball sequencing is a high throughput sequencing technology that is used to determine the entire genomic sequence of an organism. The method us...
www.ovguide.com/dna-nanoball-sequencing-9202a8c04000641f800000001ce2e7c6
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Next Generation DNA sequencing - MD Anderson Bioinformatics
Nov 23, 2010 ... first human genome sequenced over ten years at. $3 billion. ... The cost of sequencing DNA has dropped by ... Complete Genomics Nanoball.
bioinformatics.mdanderson.org/MicroarrayCourse/Lectures10/MA_DNAseq.pdf
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Overview of Sequencing Technology Platforms - Springer
Abstract The high-throughput DNA sequencing technologies are based on ... to generate long stretches of DNA that fold into nanoballs that are arrayed in the ...
www.springer.com/cda/content/document/cda_downloaddocument/9781461407812-c1.pdf?SGWID=0-0-45-1252449-p174130671
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Complete Genomics produces a cheap—well, $5,000—human ...
Nov 6, 2009 ... With the newest DNA sequencing technology starting to reach the market, we're seeing a ... The authors call these structures "DNA nanoballs.
arstechnica.com/science/2009/11/complete-genomics-produces-a-cheapwell-5000human-genome/
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DNA Nanoballs, Petabytes of Data Mark Complete Genomics ...
Oct 6, 2008 ... With the unveiling today of the next phase of the next-generation sequencing era by Complete Genomics (see accompanying story) comes ...
www.bio-itworld.com/headlines/2008/oct06/complete-genomics-dna-nanoballs.html
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DNA nanoball sequencing in science
DNA nanoball sequencing - Wikipedia, the free encyclopedia
DNA nanoball sequencing is a high throughput sequencing technology that is used to determine the entire genomic sequence of an organism. The method uses ...
DNA sequencing - Wikipedia, the free encyclopedia
Phil Green and Brent Ewing of the University of Washington described their phred quality ..... DNA nanoball sequencing is a type of high throughput sequencing ...
Complete Genomics >> Technology
Our proprietary whole human genome sequencing technology consists of the ... two primary components of our sequencing technology: DNA nanoball arrays, ...
[PDF]Overview of Sequencing Technology Platforms - Springer
Abstract The high-throughput DNA sequencing technologies are based on ... Stanford University School of Medicine , CCSR, 269 Campus Drive , ... to generate long stretches of DNA that fold into nanoballs that are arrayed in the CGA ...
[PDF]Next Generation DNA Sequencing and the Future of - MDPI.com
May 25, 2010 ... Department of Pathology, Stanford University Medical Center, 300 .... multiple linked copies of the template DNA (DNA nanoball), and the ...
[PDF]Short History of DNA Sequencing - Helsinki.fi
Lars Paulin Institute of Biotechnology University of Helsinki. Lars Paulin. DNA Sequencing. Technologies. DNA Sequencing and ..... DNA Nanoballs on array. – .
[PDF]MINI-20™ Sequencer - Azco Biotech, Inc.
synthesis) technology, exclusively licensed from Columbia University. .... Load these DNA nanoballs into a flow cell and sequence the genomic tags on the ...
[PDF]DNA Representation - InTech
The term DNA sequencing refers to methods for determining the order of the ..... However, only short sequences of DNA are determined from each DNA nanoball .... 1998 Phil Green and Brent Ewing of Washington University publish “phred” ...
Next generation sequencing - SlideShare
by Dayananda Salam, Research Scholar at Manipur University on Feb 02, 2012 ... Ion Semiconductor sequencing (Ion Torrent Systems Inc.) DNA Nanoball ( Complete ... Metagenomics – sequencing of DNA of uncultured/unpurified microbial ...
Books on the term DNA nanoball sequencing
Animal Biotechnology: Models in Discovery and Translation
Animal Biotechnology: Models in Discovery and Translation
Ashish Verma, Anchal Singh, 2013
DNA. Nanoball. Sequencing. Complete Genomics: Thereis another sequencing technology developed and commercialized by Complete Genomics, specifically optimized for humanre sequencing applications. The technology is based on a ...
Advances in Genetics, Volume 79
Advances in Genetics, Volume 79
Theodore Friedmann, Jay C Dunlap and Stephen F. Goodwin, 2012
The field of genetics is rapidly evolving and new medical breakthroughs are occuring as a result of advances in knowledge of genetics. This series continually publishes imporatnt reviews of the broadest interest to geneticists and their colleagues in affiliated disciplines. * Includes methods for testing with ethical, legal, and social implications...
Bioinformatics for High Throughput Sequencing
Bioinformatics for High Throughput Sequencing
Naiara Rodríguez-Ezpeleta, Michael Hackenberg, Ana M. Aransay, 2011
The palindrome sequences within the long linear products promote intramolecular coiling of the molecule and formation of the DNA nanoballs (DNBs ). A nanoball is a long strand of repetitive fragments of amplified DNA, which forms a ...
Prinicples of Proteomics, Second Edition
Prinicples of Proteomics, Second Edition
Richard Twyman, 2013
the sudden surge in deposited sequences from 2009 in Figure 1.1, which is remarkable considering that the y axis has a ... DNA nanoball sequencing This method uses rolling circle replication to amplify small fragments of genomic DNA into ...
DNA Sequencing:
DNA Sequencing:
Books, LLC, Source: Wikipedia, 2010
Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online.
Challenges and Opportunities of Next-Generation Sequencing for Biomedical Research, Volume 89 (Advances in Protein...
Challenges and Opportunities of Next-Generation Sequencing for Biomedical Research, Volume 89 (Advances in Protein...
Mark Rees, 2012
Published continuously since 1944, the Advances in Protein Chemistry and Structural Biology serial has been a continuous, essential resource for protein chemists. Covering reviews of methodology and research in all aspects of protein chemistry, including purification/expression, proteomics, modeling and structural determination and design, each vol...
Selected Works of Terry Speed
Selected Works of Terry Speed
Sandrine Dudoit, 2012
as 454 pyrosequencing, followed soon after by Solexa (Illumina), SOLiD, polony, DNA nanoball and Ion Torrent sequencing. As DNA sequencing technologies scaled up, huge opportunities arose along the way for the application of statistics,  ...
Advances in Endophytic Research
Advances in Endophytic Research
Vijay C. Verma and Alan C. Gange, 2013
​In recent years there has been significant attention paid on the endophytic research by various groups working within this domain. Mutualistic endophytic microbes with an emphasis on the relatively understudied fungal endophytes are the focus of this special book. Plants are associated with micro-organisms: endophytic bacteria and fungi, which liv...
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Blog posts on the term
DNA nanoball sequencing
Bitesize Bio | A Short History of Sequencing Part 2: the first of the next.
A Short History of Sequencing Part 2: the first of the next. .
bitesizebio.com/13551/a-short-history-of-sequencing-part-2-the-first-of-the-next/
DNA Nanoballs, Petabytes of Data Mark Complete Genomics Platform - Bio-IT World
With the unveiling today of the next phase of the next-generation sequencing era by Complete Genomics (see accompanying story) comes intense scrutiny of the sequencing-by-hybridization (SBH) strategy that the company says will deliver one million genome sequences in the next five years. The technology hinges on exquisite precision in manufacturing and arraying “nanoballs” of DNA as well as the ability to manage gargantuan quantities of data. Last week, Bio-IT World spoke to the two men directing the sequencing and data handling aspects of Complete Genomics’ new sequencing service. Rade Drmanac, co-founder and chief science officer of Complete Genomics, has pioneered SBH approaches in genome analysis for two decades, most noticeably co-founding HySeq (now part of Nuvelo). A decade ago it was premature, but “I think now is the right time for this technology,” he says, adding that his team has kept a belief in the advantages while exploring ways to remove the limitations. Drmanac says the new SBH platform is “a real simple high-throughput technology” that measures millions of spots of DNA in parallel. A second advantage is that the individual DNA reads are gathered independently, rather than building a chain. “Every probe is read, washed and removed, so we don’t have any chaining,” which tends to accumulate errors with the growing fragment. A Billion Nanoballs Similar to the sequencing technologies used by Applied Biosystems and the Polonator, Complete Genomics uses a ligation strategy rather than DNA polymerase. But Drmanac says the method, dubbed cPAL (combinatorial probe-anchor ligation), is based entirely on his group’s research. “It’s all [intellectual property] my team has evolved or developed in the last two years. We didn’t license any other IP,” he says. The first step is to prepare a gridded array of up to a billion DNA nanoballs, or DNBs. These DNBs are concatamers of 80-basepair (bp) mate-paired fragments of genomi
www.bio-itworld.com/headlines/2008/oct06/complete-genomics-dna-nanoballs.html
Nanoball Sequencing | electronics hobby
Nanoball Sequencing is the advanced form of DNA sequencing used to determine the entire gene sequence of an organism. The DNA nanoball sequencing technique uses the Rolling Circle model of bacterial replication to convert the DNA of genes into Nanoballs. Fluorescent probes are used to bind with the complementary sequences, then these markers are used…
dmohankumar.wordpress.com/2011/06/05/nanoball-sequencing/
Methods of DNA Sequencing~ Bioinformatics Made Simple.com
online bioinformatics blog, tools, video tutorials, basics of perl programming, jobs, conferences, PhD
www.bioinformatics-made-simple.com/2012/02/methods-of-dna-sequencing.html
CoreGenomics: Has Qiagen bought the Aldi* or Wal-Mart* of DNA sequencing companies?
This update was added on 11 July 2012: An article on GenomeWeb made interesting reading as it talked about the current litigation between Columbia University and Illumina. In it Thomas Theuringer (Qiagen PR Director) said "We support Columbia's litigation against Illumina, and are also entitled to royalties if we prevail".
core-genomics.blogspot.com/2012/06/has-qiagen-bought-aldi-or-wal-mart-of.html
DNA Sequencing
Our DNA is our genetic code - it is what makes us the way we are when it comes to our external appearance and a variety of other functions. At the heart of the
www.deoxyribosenucleicacid.com/dna/dna-sequencing/
Complete Genomics Will Sequence a Human Genome for $5000 Starting in Spring 2009
]]> Email ThisBlogThis!Share to TwitterShare to Facebook TweetAn 80-base-pair piece of DNA to be sequenced (shown here in purple) is first inserted into a circular template of DNA, along with four stretches of synthetic DNA, called adaptors (pink and blue). A specialized enzyme then makes hundreds of consecutive copies of the DNA circle.
nextbigfuture.com/2008/10/complete-genomics-will-sequence-human.html
RNA-Seq Poll Results | RNA-Seq Blog
We asked the question: Which technology will ultimately rise above all others as the choice method for high-throughput sequencing? A total of 74 RNA-Seq Blog
www.rna-seqblog.com/rna-seq-poll-results-2/
OneMedPlace – Sentinel Bioinformatics Special Report, Part I: Where Does Biotechnology Data Come From?
www.onemedplace.com/blog/archives/13259
Comparison of Next-Generation Sequencing Systems
BioMed Research International (formerly titled Journal of Biomedicine and Biotechnology) is a peer-reviewed, open access journal that publishes original research articles as well as review articles in several areas of life sciences. The journal’s Editorial Board is divided into the 55 subject areas included within the journal’s scope.
www.hindawi.com/journals/bmri/2012/251364/
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