Yellow Fluorescent Protein (YFP) is a genetic mutant of green fluorescent protein (GFP) originally derived from the jellyfish Aequorea victoria. Its excitation peak is 514 nm and its emission peak is 527 nm.
Like the parent GFP, YFP is a useful tool in cell and molecular biology thanks to its properties useful for fluorescence microscopy.
Three improved versions of YFP are Citrine, Venus, and Ypet. They have reduced chloride sensitivity, faster maturation, and increased brightness (defined as the product of the extinction coefficient and quantum yield). Typically, YFP serves as the acceptor for genetically-encoded FRET sensors of which the most likely donor FP is monomeric cyan fluorescent protein (mCFP). The red-shift relative to GFP is caused by a Pi-Pi stacking interaction as a result of the T203Y substitution introduced by mutation, which essentially increases the polarizability of the local chromophore environment as well as providing additional electron density into the chromophore.
"Venus" contains a novel amino acid substitution –F46L– which accelerates the oxidation of the chromophore at 37°C, the rate limiting step of maturation. The protein has other substitutions (F64L/ M153T/ V163A/ S175G), permitting Venus to fold well and giving it relative tolerance to acidosis and Cl-.

This is an excerpt from the article YPet from the Wikipedia free encyclopedia. A list of authors is available at Wikipedia.
The article YPet at en.wikipedia.org was accessed 7 times in the last 30 days. (as of: 11/18/2013)
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Yellow fluorescent protein - Wikipedia, the free encyclopedia
Three improved versions of YFP are Citrine, Venus, and YPet. They have reduced chloride sensitivity, faster maturation, and increased brightness (defined as ...
YPet Sequence and Map - SnapGene
YPet. Yellow fluorescent protein optimized for FRET with CPet. To obtain this DNA and protein sequence with restriction sites, please download SnapGene or  ...
Addgene: YPET-GGA3
Plasmid YPet-GGA3 from Dr. Martin Schwartz's lab contains the insert YPet- GGA3 and is published in Anal Biochem. 2008 Mar 15. 374(2):243-9. This plasmid ...
Evolutionary optimization of fluorescent proteins for intracellular ...
Feb 6, 2005 ... The resulting pair, CYPet-YPet, exhibited a 20-fold ratiometric FRET signal change, as compared to threefold for the parental pair.
YPet control : Single-molecule imaging of transcription factor binding ...
Streaming video does not reproduce the original video quality. Download the video using the link below to view at original quality. YPet fluorescent protein ...
Advances in fluorescent protein technology - Journal of Cell Science
Dec 15, 2007 ... Many of the cyan and yellow FP mutations introduced near the termini of the proteins resulted during the CYPet and YPet mutagenesis efforts ...
SuLab/YPet · GitHub
Feb 21, 2015 ... YPet - Javascript library to rapidly annotate paragraphs of text on websites with the goal of making the annotation process as fast and simple as ...
A comparison of Ypet and firefly luciferase as reporter proteins for ...
Nov 7, 2013 ... When YPet was used as a reporter protein for high-throughput screening (HTS), it showed peak fold induction and a dynamic range similar to ...
An experimental study of GFP-based FRET, with application to ...
We suspected that dimerization of the YPet–CYPet might be the problem, so we introduced the monomerizing mutation A206K (Zacharias et al. 2002), first in ...
The effect of add-on yogic prana energization technique (YPET) on ...
Mar 9, 2011 ... OBJECTIVES: The objective was to study the effect of the add-on yogic prana energization technique (YPet) on healing of fresh fractures.
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YPet in science
Type 1 University
5 Jan 2015 ... Type-1 University features a series of online courses designed to provide “higher education” to people using intensive insulin therapy (pumps ...
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We are a group of Washington State University College of Veterinary Medicine ... Many of us have experienced the loss of a pet and are here to help you through ...
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Pet Loss Support Hotline. 607-253-3932 5:30pm-8:30pm Eastern Time Every Monday, Wednesday, Thursday, and Saturday ...
Pet Loss Support Hotline - Cornell University College of Veterinary ...
Details of the pet loss hotline, with hours of availability and links to other resources.
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Books on the term YPet
Saltwater Fly Patterns
Lefty Kreh, 1995
This is the much-needed complete revision of the standard handbook on saltwater fly patterns. This new edition includes twenty new color plates and brings the total number of flies shown and described to more than 350.
No Graves As Yet: A Novel (World War I)
No Graves As Yet: A Novel (World War I)
Anne Perry, 2005
This absorbing mystery/spy thriller, set in tranquil Cambridge just before the onset of the Great War, marks a powerful start to bestseller Perry's much anticipated new series. In a lush and deceptively peaceful opening scene, college professor and chaplain Joseph Reavley is interrupted while watching a cricket game by his intelligence officer...
Handbook of Single-Molecule Biophysics
Peter Hinterdorfer, Antoine van Oijen, 2009
Nevertheless, YPet is superior for single-molecule imaging because it is the brightest YFP available, with a very high ... It was based on another bright YFP variant, Topaz [63], which was the brightest YFP before the appearance of YPet.
Methods in Bioengineering: Systems Analysis of Biological ...
Arul Jayaraman, Juergen Hahn, 2009
Amplify the CYPet and YPet genes using PCR in 25 μL reactions using primers at a final concentration of 0.1 μM and 100 ng of template. Perform PCR for 40 cycles with an annealing temperature of 59°C (the annealing temperature should be ...
Illuminating Spatiotemporal Regulation of Cyclic AMP ...
Lisa M. DiPilato, 2009
This pair, named CYPet and YPet, shows a 20- fold ratiometric FRET signal change and was designed for use in FRET-based assays (12). Upon incorporation into the ICUE design, CYPet-YPet ICUE (containing the ADEP- Epacl insert) ...
Fluorescent Proteins
Kevin F. Sullivan, 2007
Development of an Indicator for a Protein–Protein Interaction Using CYPet and YPet Caspase-activated DNase (CAD) is the primary nuclease that causes DNA fragmentation during apoptosis. In proliferating cells, CAD is expressed as an ...
Methods in Bioengineering: Systems Analysis of Biological Networks (The Artech House Methods in Bioengineering...
Methods in Bioengineering: Systems Analysis of Biological Networks (The Artech House Methods in Bioengineering...
Arul Jayaraman, 2009
This practical book is part of the new Artech House Methods in Bioengineering series volumes designed to offer detailed guidance on authoritative methods for addressing specific bioengineering challenges. Written and edited by recognized experts in the field, each book provides research engineers, scientists, and students with step-by-step procedur...
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